custom multiplex genotyping panel on a matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry platform massarray  (agena bioscience)

Journal: Nutrients

Article Title: Towards Precision Sports Nutrition for Endurance Athletes: A Scoping Review of Application of Omics and Wearables Technologies

doi: 10.3390/nu16223943

Figure Lengend Snippet: Summaries of nutrigenetics studies’ characteristics and key findings in endurance athletes.

Article Snippet: Guest et al., 2022 [ ] , Whole-plot complete randomized block, double-blinded, placebo-controlled design with 3 supplementation days, 1 week apart; saliva sample time point: before the intervention , 100 competitive male athletes; HTR2A rs6313 CC genotype (mean age: 24 ± 4 years, VO 2 max: 49 ± 8 mL kg −1 min −1 ), CT genotype (mean age: 25 ± 5 years, VO 2 max: 47 ± 12 mL kg −1 min −1 ), TT genotype (mean age: 25 ± 5 years, VO 2 max: 44 ± 12 mL kg −1 min −1 ) , Genotyping (Sequenom MassArray platform, Sequenom Inc., San Diego, CA, USA), target SNPs: HTR2A rs6313, CYP1A2 rs762551; saliva , Participants received placebo or caffeine (2 mg/kg or 4 mg/kg) before a 10 km cycling time trial. , 4 mg/kg caffeine ↑ performance in individuals with both the HTR2A CC and CYP1A2 AA genotypes. Among CYP1A2 AA individuals, HTR2A CC genotypes outperformed T-allele carriers. No performance differences in CYP1A2 C allele carriers based on HTR2A genotype..

Techniques: Control, Blocking Assay